Tumour necrosis factor-alpha polymorphism -308 G/a and its protein in subjects with gingivitis.

OBJECTIVE: This study examined the association between tumour necrosis factor-alpha (TNF- α) (-308 G/A) polymorphism and gingivitis, and serum and salivary TNF- α levels, in a Mexican population. MATERIAL AND METHODS: This study enrolled 171 subjects, divided into two groups: healthy subjects and gingivitis patients. TNF- α (-308 G/A) gene polymorphism was analyzed by PCR-RFLP assay. Salivary and serum samples were used to measure cytokine levels through the ELISA technique. RESULTS: TNF- α (-308 G/A) polymorphism was shown to have a protective effect in carriers of the A/A genotype and allele A. The G/A genotype is associated with an increase in high-density lipoprotein cholesterol (HDL-C) levels in the gingivitis group. Healthy individuals had higher levels of salivary TNF- α and HDL-C, and increased salivary flow. Triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels were increased in the gingivitis group. No statistical differences were found in serum TNF- α levels. CONCLUSION: Our data demonstrate that the TNF- α -308 A/A genotype exerts a protective effect against gingivitis. Moreover, oral conditions are associated with some biochemical parameters.

Association of Obesity with Depressive Symptomatology, Eating Habits, Interleukin-8 and Cortisol in a Young Population.

Obesity is the result of a complex combination of psychological, biological, and environmental factors. In this work, we evaluate whether obesity is related to eating habits, depressive symptomatology, as well as interleukin-8 and cortisol. A descriptive cross-sectional study was carried out in 232 university students. All youths were surveyed to determine their eating habits and depressive symptomatology. Anthropometric measures and a blood sample were taken to determine its biochemical profile and its concentration of interleukin-8 and cortisol. The results show that interleukin-8 increase in the overfat group. The altered eating behaviors were frequent in the studied group; they were associated with the presence of obesity and the variation of interleukin-8 and cortisol. Besides, we found correlations of interleukin-8 with age, glucose, and lipid profile in the overfat group. In conclusion, these results indicate that high adiposity is related to changes in the concentrations of interleukin-8 and eating habits, confirming that obesity is the consequence of a complex network of various factors.

Alteration of cytokines in saliva of children with caries and obesity.

Caries and obesity are multifactorial diseases with inflammatory components, whose processes involve cells and molecules, such as cytokines. Therefore, the objective of this work was to compare the concentrations of IL-6, IL-8, IL-15, and IL-18 in the salivary samples of children with caries and obesity. The study was carried out with 80 children: 43 with a normal weight and 37 with obesity. The diagnosis of caries was carried out using the ICDAS system. Salivary samples were used to measure the cytokine levels via the ELISA technique. Our results show that children with obesity and dental cavities have high levels of IL-6 and IL-15. Similarly, obese children have elevated levels of these two cytokines, while children with cavitated carious lesions presented alterations in their concentrations of IL-6 and IL-8. In conclusion, our data suggest that IL-6 has a significant effect on both obesity and caries, although IL-8 is more related to caries, and IL-15 is more related to obesity.

Consulta por extracción dental en niños nicaragüenses: una aproximación a las necesidades de atención bucal

Objetivo: Identificar los factores asociados a las visitas al dentista por motivo de extracción dental en niños nicaragüenses. Material y Métodos: Se realizó un estudio transversal en una muestra de escolares de 6 a 12 años de edad. Se incluyeron 1400 niños de escuelas públicas, a cuyos padres/tutores se les aplicó un cuestionario para determinar una serie de variables sociodemográficas, socioeconómicas y dentales. La variable dependiente fue la visita al dentista por extracción dental (VDED), la cual fue categorizada como: 0=Sin visita al dentista, 1= VDED, pero no en el último año, 2= VDED en el último año. En el análisis estadístico se emplearon pruebas estadísticas no paramétricas. Resultados: El promedio de edad fue de 9.00±2.00 años y las mujeres representaron el 49.9%. Del total de la muestra, 21.6% acudieron al dentista por extracción dental, de los cuales 9.8% lo hicieron hace más de un año y 11.8% durante al año pasado previo a la encuesta. Las variables que mostraron diferencias significativas (p<0.05) en el análisis estadístico de las VDED fueron la edad, la edad de la madre, y la posición socioeconómica. Conclusiones: Uno de cada cinco niños tuvo VDED. Las visitas al dentista por extracciones dentales podrían utilizarse como un indicador del estado de salud bucal de la población, ya que son una aproximación a las necesidades de salud que presentan.

Objective: To identify factors associated with visits to the dentist for dental extraction in Nicaraguan children. Materials and Methods: This was a cross-sectional study that was carried out in a sample of schoolchildren from 6 to 12 years of age. We included 1400 children from public schools, whose parents / guardians were given a questionnaire to determine a series of sociodemographic, socioeconomic and dental variables. The dependent variable was defined as a dental visit for dental extraction (VDED), which was categorized as: 0 = No visit to the dentist, 1 = VDED, but not in the last year, 2 = VDED in the last year. For statistical analysis, nonparametric statistical tests were used. Results: The average age was 9.00 ± 2.00 years, 49.9% of the total were females. Of the total sample, 21.6% went to the dentist for a dental extraction, of which 9.8% did so more than a year ago and 11.8% had gone during the year prior to the survey. The variables that showed significant differences (p <0.05) in the statistical analysis of the VDED were age of the patient, the age of the mother, and the socioeconomic status. Conclusions: One in five children had VDED. Visits to the dentist for dental extractions could be used as an indicator of the oral health status of the population, since they are an approximation the other health needs present in the population.